%0 Journal Article
%T Construction of pGL3-SM22-SCAP (D443N) eukaryotic expression vector and its expression in CHO cells<br>SM22启动SCAP真核表达质粒的构建及其在CHO细胞中的表达
%A Yuanyuan Wang
%A Jieli Hu
%A Jing Cui
%A Ailong Huang
%A Xiongzhong Ruan
%A Yaxi Chen
%A <br>王媛媛
%A 胡接力
%A 崔静
%A 黄爱龙
%A 阮雄中
%A 陈压西
%J 生物工程学报
%D 2010
%I 
%X The experiment was designed to investigate the function of SREBP cleavage-activating protein (SCAP) mutant (D443N) by constructing an eukaryotic expressive vector using a smooth muscle specific promoter SM22 (pGL3-SM22-SCAP(D443N)). SM22 promoter (pSM22) was amplified from genome DNA of mice by nested PCR, and then cloned into pMD-T vector. The SM22 promoter fragment released from the vector by Kpn I and Hind III digestion was sub-cloned into pGL3-control-Luc vector, to form pGL3-SM22-Luc. The activity of p...
%K SM22
%K promoter
%K SREBP cleavage-activating protein
%K plasmid construction
%K gene expression
%K transfection<br>SM22
%K 启动子
%K SREBP裂解激活蛋白
%K 质粒构建
%K 基因表达
%K 转染
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=D7F57D3B8D195A1C1F6214FFA2483B1C&yid=140ECF96957D60B2&vid=96C778EE049EE47D&iid=CA4FD0336C81A37A&sid=DDD31293A7C7D057&eid=2B5DE8A23DCEED39&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=0