%0 Journal Article
%T Analysis of Rice OsPLD3 and OsPLD4 Genes and Promoters<br>水稻OsPLD3和OsPLD4基因及其启动子
%A Kun Yang
%A Yaping Fu
%A Yuman Zhang
%A Yongsheng Yan
%A Zhiqiang Zhao
%A Rongxiang Fang
%A Zongxiu Sun
%A Xiaoying Chen
%A <br>杨 鹍
%A 傅亚萍
%A 张玉满
%A 颜永胜
%A 赵志强
%A 方荣祥
%A 孙宗修
%A 陈晓英
%J 生物工程学报
%D 2008
%I 
%X Phospholipase Ds (PLDs) exist in many plants. PLDs catalyse the hydrolysis of phospholipids (e.g. phosphatidylcholine) in cell membrane into phosphatidic acid (PA) and polar free heads (e.g. choline). Two PLD members from rice, OsPLD3 and OsPLD4, were studied by reverse genetics approaches. The results showed that the promoters of OsPLD3 and OsPLD4 could drive the expression of the reporter gene in various tissues of the rice flower organs at different levels. The expression of both genes was induced by wounding and methyl jasmonate (MeJA), but with different intensity at different time intervals. No prominent phenotypes were observed by RNA interference with the gene-specific artificial miRNAs or over-expression of the target genes in rice plants, implying the functional redundancy among different members of the rice PLD family.
%K OsPLD
%K wounding induction
%K methyl jasmonate
%K flower organ<br>水稻磷脂酶D
%K 损伤诱导
%K 茉莉酸甲酯
%K 花器官
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=D7CACEC0250C94307D4FBFA89874D65D&yid=67289AFF6305E306&vid=B91E8C6D6FE990DB&iid=38B194292C032A66&sid=869B6F3117981EC4&eid=315A3D008C6ECFC8&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=22