%0 Journal Article
%T Analysis of microbial diversity of nitrifying bacteria by Terminal Restriction Fragment Length Polymorphism
末端限制性片段长度多态性技术分析硝化细菌微生物多样性
%A Weitie Lin
%A Yanan Zhu
%A
林炜铁
%A 朱雅楠
%J 生物工程学报
%D 2010
%I
%X We analyzed the microbial diversity and quantity of nitrifying bacteria in the enrichment reactor by Terminal Restriction Fragment Length Polymorphism (T_RFLP), a cultured-independent molecular technique. The result indicated that nitrobacteria enriched the best, and the diversity index decreased 62.80% compared with the initial data. Nitrobacteria were predominant in the reactor. Meanwhile, we studied the microbial diversity before and after adding Nitrobacteria into shrimp ponds, and analyzed several major bacterial species that existed stably in the pond. According to the analysis by T_RFLP program, species including Brevibacillus brevis, Microbacterium lactium, Azoarcus indigens and Bordetella holmesii were the dominant bacteria in the ponds.
%K Terminal Restriction Fragment Length Polymorphism
%K nitrobacter
%K reactor
%K microbial community structure
%K microbial diversity
T_RFLP,硝化细菌,反应器,群落结构,微生物多样性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=4F1EA5970387CEED4894351C1A7B292B&yid=140ECF96957D60B2&vid=96C778EE049EE47D&iid=E158A972A605785F&sid=07C6E4664BB7C5DA&eid=8CE1095CD639AEF4&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=5