%0 Journal Article
%T Preparation and detection of phosphorylated PRAS40 (Ser183) polyclonal antibody<br>PRAS40(Ser183)磷酸化多克隆抗体的制备及检测
%A Hao Wei
%A Bei Huang
%A Changzhi Xu
%A Zhuxia Zheng
%A Yu Bai
%A <br>魏皓
%A 黄蓓
%A 徐昌志
%A 郑竹霞
%A 白宇
%J 生物工程学报
%D 2009
%I 
%X PRAS40 (proline-rich Akt substrate 40 kD) associates with mammalian target of rapamycin complex 1(mTORC1), serine 183 site (Ser183) of PRAS40 can be phosphorylated by mTORC1. To prepare the phosphorylated PRAS40 (Ser183) antibody, We chosen 10-amino acid including Ser183 as antigen peptide through antigenicity and hydrophobicity analysis, hinged on keyhole limpet hemocyanin (KLH), and used the KLH-peptide to immunize rabbits. After antibody serum titer detection by enzyme linked immunosorbent assay (ELISA),...
%K PRAS40 (Ser183)
%K phosphorylated polyclonal antibody
%K rProtein A affinity chromatography
%K amino acid withdraw<br>PRAS40（Ser183）
%K 磷酸化抗体
%K rProteinA亲和层析
%K 饥饿处理
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=55BB320DD98B7932579D228791D6FD63&yid=DE12191FBD62783C&vid=C5154311167311FE&iid=DF92D298D3FF1E6E&sid=AE9D5375B9B2D7F2&eid=1A775ABEB80E436B&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=7