%0 Journal Article
%T Stable expression of rhVEGF165 in Chinese hamster ovary cells<br>rhVEGF165在中国仓鼠卵巢细胞中的稳定表达、纯化及其生物学活性
%A Ganliang Zhang
%A Haifeng Zhang
%A Weihong Nian
%A Jiuru Sun
%A <br>张甘良
%A 张海峰
%A 粘伟红
%A 孙九如
%J 生物工程学报
%D 2011
%I 
%X We established a stable Chinese hamster ovary (CHO-S) cell line for recombinant human VEGF165-expressing. We co-transfected GS-expression vector and rhVEGF165 expression plasmid into CHO-S cells, and selected the highest VEGF165- expressing clone as the working cell line to express VEGF165 protein. After 7-day fed-batch culture in a 5 L bioreactor and 3 steps chromatographic purification, we got the rhVEGF165 protein for series of binding and biological activity examination. The production was over 50 mg/L. The purified rhVEGF165 protein was functionally active with a half-maximal Human Umbilical Vein Endothelial Cells (HUVEC) growth-enhancing effect concentration of 1.94 ng/mL. It was slightly better than commercially available Escherichia coli expressing rhVEGF165. So we expressed successfully rhVEGF165 protein in high-level and obtained the fully active rhVEGF165 protein in large quantity.
%K rhVEGF165
%K Chinese hamster ovary (CHO) cell
%K stable expression
%K bioreactor process
%K rhVEGF165 purification<br>血管内皮生长因子
%K (VEGF165)，中国仓鼠卵巢细胞，重组蛋白稳定表达，生物反应器，rhVEGF165蛋白纯化
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=9880CD65C317F63D0C47D5C1F4436318&yid=9377ED8094509821&vid=DB817633AA4F79B9&iid=B31275AF3241DB2D&sid=073C3CF5F13F64FE&eid=72D13CE6C162BDF4&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=0