%0 Journal Article
%T Effect of overexpression of nicotinic acid phosphoribosyl transferase on succinic acid production in Escherichia coli NZN111<br>过量表达烟酸转磷酸核糖激酶对大肠杆菌NZN111产丁二酸的影响
%A Rongming Liu
%A Jiangfeng M
%A Liya Liang
%A Bing Xu
%A Guangming Wang
%A Min Zhang
%A Min Jiang
%A <br>刘嵘明
%A 马江锋
%A 梁丽亚
%A 徐冰
%A 王光明
%A 张敏
%A 姜岷
%J 生物工程学报
%D 2011
%I 
%X Escherichia coli strain NZN111 is a promising candidate for the fermentative production of succinate. However, because lactate dehydrogenase and pyruvate formate lyase were inactivated in NZN111, this strain had an unbalanced NADH/NAD+ ratio and could not use glucose under anaerobic conditions. In this study, a recombinant strain E. coli NZN111/pTrc99a-pncB was constructed to overexpress the nicotinic acid phosphoribosyl transferase gene (pncB). Under anaerobic conditions with the addition of 0.5 mmol/L nicotinic acid and 0.3 mmol/L isopropyl beta-D-thiogalactopyranoside (IPTG), the specific nicotinic acid phosphoribosyl transferase (NAPRTase, EC 2.4.2.11) activity in the recombinant strain was 11-fold higher than that in E. coli NZN111, the concentration of NAD(H) was increased by 3.85-fold, especially the concentration of NAD+ was increased by 5.17-fold and NADH/NAD+ was decreased from 0.640 to 0.125. The recombinant strain regained the capability of growth and glucose utilization under anaerobic conditions.
%K nicotinic acid phosphoribosyl transferase
%K Escherichia coli NZN111
%K succinic acid
%K dual-phase fermentation<br>烟酸转磷酸核糖激酶，大肠杆菌NZN111，丁二酸，两阶段发酵
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=F437B1BF01E7F486FE19BEF44FA6CFB9&yid=9377ED8094509821&vid=DB817633AA4F79B9&iid=F3090AE9B60B7ED1&sid=5A66347DEC1DE6F4&eid=6EAE3EAEEC5D0463&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=22