%0 Journal Article
%T Application of microfluidics in cell transfection: a review<br>微流控技术在细胞转染中的应用
%A Haiming Xu
%A Jiahuan Jiang
%A <br>徐海明
%A 蒋稼欢
%J 生物工程学报
%D 2011
%I 
%X Microfluidics deals with the manipulation of fluidics in the structure with dimensions of micrometers or nanometers. As an emerging field, microfluidics has numerous advantages, such as controllable fluid flow and reduced reagents consumption. Recently, microfluidic has been applied into the area of cell transfection, providing opportunities to investigate cell transfection process on microscale. This review summarizes recent technical development of cell transfection based on microfluidics, including transfeceted microarray, transfecetion established in miniaturization flowing space, microdrops, microinjection and microfluidic electroporation. The factors that affect the transfection efficiency and improvement approaches are also discussed.
%K microfludic
%K transfection
%K gene
%K efficiency<br>微流控，转染，基因，效率
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=F437B1BF01E7F486549FE9ADBA8726E1&yid=9377ED8094509821&vid=DB817633AA4F79B9&iid=F3090AE9B60B7ED1&sid=656BC79BFC7F0F4B&eid=83F3E1555B654B95&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=58