%0 Journal Article
%T Immunogenicity and antigenicity of Japanese encephalitis virus envelope protein domain III<br>日本脑炎病毒SA14-14-2 E蛋白结构域Ⅲ的抗原性和免疫原性分析
%A Ying Huang
%A Shan Liu
%A Peng Yang
%A Yun Du
%A Zhiwei Sun
%A Weiyuan Yu
%A <br>黄莺
%A 刘珊
%A 杨鹏
%A 杜韫
%A 孙志伟
%A 俞炜源
%J 生物工程学报
%D 2009
%I 
%X To express the domain III gene of Japanese encephalitis virus (JEV) and to learn the possibility of developing the DIII protein as a subunit vaccine, we amplified the JEV DIII gene by PCR and constructed the expression plasmid pET-JE DIII by inserting JEV DIII gene into the prokaryotic expression vector pET-32a(+). The domain III protein of the attenuated strain SA14-14-2 was expressed as a thioredoxin (Trx) fusion protein, which was unique in forming a large fraction of the soluble recombinant protein. We ...
%K Japanese encephalitis virus(JEV)
%K E protein
%K domain III
%K subunit vaccine<br>日本脑炎病毒
%K E蛋白
%K DIII区
%K 亚单位疫苗
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=81E128FD279612E583536F482D042F5C&yid=DE12191FBD62783C&vid=C5154311167311FE&iid=F3090AE9B60B7ED1&sid=B9B1149CB26916EC&eid=05D0CF7AA2D40B14&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=0