%0 Journal Article
%T Improving infection efficiency of adult cynomolgus monkey mesenchymal stem cells with lentiviral vectors<br>慢病毒载体感染成年食蟹猴骨髓间充质干细胞
%A Zhenhua Ren
%A Shuyan Wang
%A Ying Zhang
%A Chunlin Zou
%A Y Alex Zhang
%A <br>任振华
%A 王淑艳
%A 张颖
%A 邹春林
%A 张愚
%J 生物工程学报
%D 2010
%I 
%X Mesenchymal stem cells (MSCs) have received considerable attention for various therapeutic approaches in recent years. MSCs are also easy to genetically modify to express therapeutic genes by using lentiviral vectors. Because of the similarities in genetics, physiology and metabolism between non-human primates (NHPs) and humans, NHPs models are invaluable for researching human disorders and for developing therapeutic strategies. Therefore, MSCs derived from NHPs could be a powerful tool for cell therapy and genetic engineering. Studies from captive and free-ranging adult NHPs show that up to 100% were infected with simian foamy virus (SFV). In this study, we found that all cultured MSCs derived from adult cynomolgus monkey were infected with SFV by RT-PCR. Therefore, antiviral drugs must be added in MSCs culture. However, because of SFV infection and additive antiviral drugs, the infection efficiency of the lentiviral vectors reduced significantly. In this study, we improved the infection efficiency by disabled antiviral drugs before lentiviral infection. It might be provide technical assistance for the culture of adult cynomolgus monkey MSCs as genetically engineered cells applied to clinical and experimental research.
%K cynomolgus monkey mesenchymal stem cells
%K green fluorescent protein
%K lentiviral vectors
%K simian foamy viruses
%K transduction<br>食蟹猴骨髓间充质干细胞，绿色荧光蛋白，慢病毒载体，猴泡沫病毒，转导
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=04B08312DABFDF53675E8F81D9411259&yid=140ECF96957D60B2&vid=96C778EE049EE47D&iid=B31275AF3241DB2D&sid=7B927C26AC9ED104&eid=FC6FCA5A7559F1FB&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=1&reference_num=0