%0 Journal Article
%T Recombinant Vp2 protein of infectious bursal disease virus AH1 strain expressed in insect cells: a vaccine candidate<br>传染性法氏囊病病毒AH1株vp2基因在昆虫细胞中的表达与应用
%A Wei Ouyang
%A Yongshan Wang
%A Yu Zhou
%A Haibin Zhang
%A Yude Tang
%A <br>欧阳伟
%A 王永山
%A 周宇
%A 张海彬
%A 唐雨德
%J 生物工程学报
%D 2010
%I 
%X Protective immune response of the available IBD vaccine is insufficient to fully protect against the prevailing strain of the infectious bursal disease virus (IBDV). Such a vaccination escape IBDV field isolate idenfied from Anhui province of China in December 2007, where IBD broke out at 2 weeks post vaccination. The IBDV vp2 gene was cloned into pFastBacHTA donor plasmid, followed by generation of the recombinant bacmid DNA pBac-VP2. The latter was used to transfect insect cell Sf9 with Lipofectamine to p...
%K infectious bursal disease virus
%K vp2 gene
%K virus-like particles (VLPs)
%K inclusion body-like structure
%K indirect ELISA
%K protection test<br>传染性法氏囊病病毒，vp2基因，病毒样颗粒，包涵体样结构，间接ELISA，免疫实验
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=1D117BC346650E74224DEE85E48BEB44&yid=140ECF96957D60B2&vid=96C778EE049EE47D&iid=94C357A881DFC066&sid=240CB58995465C01&eid=70E3F4DEB0172F14&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=0