%0 Journal Article %T Kinetics of enzymatic hydrolysis of steam-explosion pretreated corn straw
蒸汽爆破玉米秸秆酶解动力学 %A Ren Shengtao %A Cheng Keke %A Song Andong %A Zhang Jian'an %A
任省涛 %A 程可可 %A 宋安东 %A 张建安 %J 生物工程学报 %D 2011 %I %X In order to learn the enzymatic hydrolysis characteristics of steam-explosion pretreated corn straw by cellulase, the effects of substrate concentration, cellulase concentration and temperature were determined. The kinetics of the hydrolysis reaction could be described with the Michealis-Menten equation, and the hydrolysis reaction obeyed the classical first-order reaction rate in the first three hours. In the condition of 45 °C and pH 5.0 and the stirring rate 120 r/min, the Michealis constant (Km) and maximum rate (Vm) for 1.2 FPU/mL of cellulase were 11.71 g/L and 1.5 g/(L·h). The kinetic model, including the parameters such as substrate concentration, enzymatic concentration and temperature, was suit for the hydrolysis reaction under the temperature range from 30 °C?50 °C. %K Michealis-Menten equation %K corn stover %K enzymatic hydrolysis %K kinetics %K cellulase
米氏方程,玉米秸秆,酶解,动力学,纤维素酶 %U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=D1E202F235432DF816217D3DE509FDD5&yid=9377ED8094509821&vid=DB817633AA4F79B9&iid=E158A972A605785F&sid=106103EB0EA31435&eid=0A8675156EB60B87&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=20