%0 Journal Article
%T Asymmetric synthesis of atorvastatin intermediate by Pichia pastoris X-33<br>毕赤酵母不对称合成阿托伐他汀中间体
%A Jianping Zhou
%A Yuhong Ren
%A Minjie Zhang
%A Xiaofeng Sun
%A Dongzhi Wei
%A <br>周剑平
%A 任宇红
%A 张敏洁
%A 孙晓锋
%A 魏东芝
%J 生物工程学报
%D 2011
%I 
%X Ethyl (R)-3-hydroxy-5-(1,3-dioxoisoindolin-2-yl)-pentanoate is a potential intermediate for the synthesis of HMG-CoA reductase inhibitor (atorvastatin) that can lower the cholesterol level in human blood. In this study, in order to synthesize ethyl (R)-3-hydroxy-5-(1,3-dioxoisoindolin-2-yl)-pentanoate by bioreduction, the yeast strains in our lab were screened. Ethyl (R)-3-hydroxy-5-(1,3-dioxoisoindolin-2-yl)-pentanoate was found to be produced efficiently from ethyl 5-(1,3-dioxoisoindolin-2-yl)-3-oxopentanoate by Pichia pastoris X-33. The effects of initial substrate concentration, reaction time, co-substrate, amount of yeast cells, pH, as well as the temperature on the yield and enantiomeric excesses (e.e. value) of product were examined in mono-phase system. The optimal reaction conditions are as fallows: substrate concentration 7 g/L, cell concentration 120 g/L, glucose concentration 120 g/L, pH 6.5, temperature 35 °C, reaction time 12 h, and the yield 93.12% with the high e.e. value of 98.55%.
%K Pichia pastoris X-33
%K asymmetric reduction
%K ethyl 5-(1
%K 3-dioxoisoindolin-2-yl)-3-oxopentanoate
%K ethyl (R)-3-hydroxy-5-(1
%K 3-dioxoisoindolin-2-yl)- pentanoate<br>毕赤酵母，不对称还原，5-邻苯二甲酰亚胺-3-氧代戊酸乙酯，(R)-3-羟基-5-邻苯二甲酰亚胺基戊酸乙酯
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=A5B2A8BDD56590EBC3A28C68674372DB&yid=9377ED8094509821&vid=DB817633AA4F79B9&iid=E158A972A605785F&sid=57EA20F731155703&eid=7882A2973AA04DE8&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=7