%0 Journal Article
%T Preparation and properties of SiO2 tubes immobilized antibody for HCAg detection<br>用于丙型肝炎病毒抗原测定的SiO2载体的制备及其性质分析
%A Li Xie
%A Yueping Guan
%A Ying Ge
%A Hongbo Shi
%A <br>谢立
%A 官月平
%A 戈莹
%A 时洪波
%J 生物工程学报
%D 2010
%I 
%X In order to optimize the fabrication of SiO2 tubes immobilized with antibody for hepatitis C virus antigen (HCAg) detection, we formed the activated amino on the surface of SiO2 tubes by using the activation of aminosilane. Then we immobilized the hepatitis C virus (HCV) monoclonal antibody on the surface of SiO2 tubes by using glutaraldehyde as a chemical cross-linker, followed by detecting HCAg. Sequence tests showed that when the SiO2 tubes were treated in 10% (V/V) aminosilane solution and 3% (V/V) glutaraldehyde solution for 3 hours and 2 hours, respectively, the HCV monoclonal antibody had high immobilization efficiency and low nonspecificity, and the HCAg was detected to 1 ng/mL. This experiment can provide principle and experimental data for establishment of HCAg magnetic immunoassay system.
%K hepatitis C virus antigen
%K HCV monoclonal antibody
%K covalent immobilization
%K aminosilane
%K glutaraldehyde
%K SiO2 tubes<br>丙肝病毒抗原，丙肝单克隆抗体，共价交联，氨基硅烷，戊二醛，SiO2载体
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=2370A36913A52308208657E2D1459C4F&yid=140ECF96957D60B2&vid=96C778EE049EE47D&iid=E158A972A605785F&sid=6313C162FF75889A&eid=811ACA5D3673A764&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=0