%0 Journal Article
%T Expression of anti-avian influenza virus H5N1 secretory IgA in Chinese hamster ovary cells<br>抗禽流感病毒H5N1分泌型IgA在中国仓鼠卵巢细胞中的表达
%A Cun Li
%A Baozhong Zhang
%A Xiaoping An
%A Zhiqiang Mi
%A Dabin Liu
%A Huanhuan Jiang
%A Bo Pan
%A Sheng Wang
%A Bin Chen
%A Fen Huang
%A Juan Wang
%A Xiaona Wang
%A Yigang Tong
%A <br>李存
%A 张宝中
%A 安小平
%A 米志强
%A 刘大斌
%A 姜焕焕
%A 潘博
%A 王盛
%A 陈斌
%A 黄芬
%A 王娟
%A 王晓娜
%A 童贻刚
%J 生物工程学报
%D 2011
%I 
%X Secretory IgA (SIgA) antibodies in external secretions playing an important role in mucosal immune response. Polymeric SIgA was advantageous over monomeric IgA (mIgA) and IgG in several aspects. To express secretory IgA antibody against H5N1 virus, we constructed the secretory component and immunoglobulin J expressing plasmids and co-transfected the plasmids into the Chinese hamster ovary cells (CHO) stably expressing immunoglobulin A. Then we used Zeocin to select the positive clone cells, monoclonal cells stably secreting SIgA was screened through fold dilution method at last. The SIgA antibody secreted from the CHO cells was confirmed by Western blotting, which demonstrated that we had got the complete SIgA molecular. The successful expression of this polymeric anti-H5N1 SIgA in CHO cells will contribute to the production of recombinant SIgA as a preventive agent for infectious disease control.
%K Avian influenza virus H5N1
%K Western blotting
%K secretory Immunoglobulin A<br>禽流感病毒H5N1，Western
%K blotting，SIgA抗体
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=3EEA3AA8AEC7D3C37BE0F310332BB504&yid=9377ED8094509821&vid=DB817633AA4F79B9&iid=0B39A22176CE99FB&sid=D5C73DEF4CF8FAF3&eid=4966445AEEBA9556&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=10