%0 Journal Article
%T Differential Expression of Haemolymph Proteome of Resistant Strain and Susceptible Strain for BmNPV in Bombyx mori L.<br>家蚕抗BmNPV品系与感性品系血淋巴液蛋白质组的差异分析
%A Keya Cai
%A Keping Chen
%A Xiaoyong Liu
%A Qin Yao
%A Jun Li
%A <br>蔡克亚
%A 陈克平
%A 刘晓勇
%A 姚勤
%A 李军
%J 生物工程学报
%D 2008
%I 
%X Three model silkworms, highly resistant strain, highly susceptible strain and their near isogenic line were established by hybridization and backcross. The resistance of silkworm (Bombyx mori L.) to BmNPV was studied at proteomic level using two-dimensional gel electrophoresis and MALDI TOF/TOF MS. Differential expression profiles of proteome in resistant strain, susceptible strain and near isogenic line were obtained, and 180, 190 and 187 of protein spots were shown, respectively. Among them, 80% were concentrated in pI 5~9. Twelve differential protein spots in total were obtained from 3 gels. Using MALDI TOF/TOF MS, five proteins, including aminoacylase, were identified from these spots. The exclusive expression of aminoacylase in highly resistant strain and near isogenic line and its absence in susceptible strain suggest that it might be a BmNPV-resistance related protein.
%K two-dimensional gel electrophoresis
%K MALDI TOF/TOF MS
%K proteome
%K resistibility to BmNPV
%K silkworm Bombyx mori L
%K )<br>2-D电泳
%K MALDI
%K TOF/TOF
%K MS
%K 蛋白质组
%K 抗核型多角体病毒
%K 家蚕
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=48CA2A5C711801356BDB5572657259E4&yid=67289AFF6305E306&vid=B91E8C6D6FE990DB&iid=0B39A22176CE99FB&sid=B8F8200D88DDC7D6&eid=211C7E02AC474301&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=22