%0 Journal Article
%T Progress in expression and molecular modification of microbial transglutaminase<br>微生物谷氨酰胺转胺酶的表达及分子改造研究进展
%A Song Liu
%A Dongxu Zhang
%A Guocheng Du
%A Jian Chen
%A <br>刘松
%A 张东旭
%A 堵国成
%A 陈坚
%J 生物工程学报
%D 2011
%I 
%X Microbial transglutaminase, which could catalyze the cross-linking of many proteins or non-protein materials, has been widely used in food, pharmaceutical and textile industry. To enhance the yield of the enzyme and establish corresponding platform for molecular modification, the researchers of Japanese Ajinomoto began to construct the recombinant strain producing transglutaminase in the 1990s. So far, the enzyme has been successfully expressed in different expression systems. Some of the recombinant strains are more productive than wild strains. Recently, progress has been made in the molecular modification of microbial transglutaminase, and the activity, thermo-stability and specificity of the enzyme are improved. This review briefly summarized and analyzed the strategies involved in these studies, and noted its trends.
%K Streptomyces
%K transglutaminase
%K protein expression
%K molecular modification
%K protein structure<br>链霉菌
%K 谷氨酰胺转胺酶
%K 蛋白质表达
%K 分子改造
%K 蛋白质结构
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=4D541C05C7575AEA959C74E32904DF3A&yid=9377ED8094509821&vid=DB817633AA4F79B9&iid=59906B3B2830C2C5&sid=B219870B99929345&eid=4858DFA42406A0F9&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=48