%0 Journal Article
%T Improved lipid productivity of Nannochloropsis by heavy-ion irradiation mutagenesis<br>重离子诱变创制高产油微拟球藻新品种
%A Zhiyao Wang
%A Yubin M
%A Runzhi Mu
%A Changjiang Sun
%A Dongyuan Zhang
%A Yongfei Wang
%A <br>王芝瑶
%A 马玉彬
%A 牟润芝
%A 孙长江
%A 张东远
%A 王永飞
%J 生物工程学报
%D 2013
%I 
%X Nannochloropsis has been considered as a promising feedstock for biodiesel production in recent years. To improve its lipid productivity, heavy-ion irradiation mutagenesis, an effectively breeding method used in plants and microorganisms was applied in Nannochloropsis oceanica OZ-1. After large-scale screening using Imaging-PAM and microplate-reader, two mutants (HP-1 and HP-2) with higher growth rate were isolated from the wild type N. oceanica. Subsequently analysis showed that after 18 days of cultivation biomass accumulation of the HP-1 and HP-2 mutant was increased by 19% and 26% respectively compare to the wild type. Total lipid productivity of the HP-1 and HP-2 mutant was 295 mg/(L·d) and 275 mg/(L·d), respectively, whereas that of the wild type was 247 mg/(L·d). Both mutants showed significantly advantage over their wild type concerning biomass accumulation and lipid productivity.
%K Nannochloropsis oceanica OZ-1
%K heavy-ion irradiation mutagenesis
%K microalgal biodiesel<br>微拟球藻，重离子诱变，微藻生物柴油
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=2DB3B6D3544EDEBD33EF6291888C3D2D&yid=FF7AA908D58E97FA&vid=771469D9D58C34FF&iid=CA4FD0336C81A37A&sid=EFD65B51496FB200&eid=B62E0EEFE746E568&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=0