%0 Journal Article
%T Recombinant goat pox virus expressing PPRV H protein<br>表达小反刍兽疫H蛋白的重组山羊痘病毒疫苗
%A Weiye Chen
%A Linmao Qu
%A Sen Hu
%A Qianqian Hu
%A Qian Zhang
%A Haibing Zhi
%A Kehe Huang
%A Zhigao Bu
%A <br>陈伟业
%A 曲林茂
%A 胡森
%A 胡倩倩
%A 张倩
%A 支海兵
%A 黄克和
%A 步志高
%J 生物工程学报
%D 2009
%I 
%X The purpose of the study is to construct recombinant goat pox virus (GPV) expressing Peste des petits ruminants virus (PPRV) H protein,and to evaluate the immunization effect. Recombinant GPV containing PPRV H gene (rGPV-PPRV-H) was selected and purified by gpt and eGFP utilizing plaque purification,and the final selected recombinant GPV was proved to be purified by PCR. Immunofluorescence and Western blotting showed that the recombinant virus could express H protein of PPRV while infecting lamb testis cell...
%K recombinant vaccine
%K Peste des petits of ruminants (PPR)
%K goat pox virus vector
%K neutralization antibodies<br>重组疫苗
%K 小反刍兽疫
%K 山羊痘病毒载体
%K 中和抗体
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=A114768C530CC5EEB2882747019C4AC5&yid=DE12191FBD62783C&vid=C5154311167311FE&iid=E158A972A605785F&sid=C4490A71BEB872FA&eid=51F9E747BA1ACB45&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=9