%0 Journal Article
%T Cloning of bovine c-myc gene and its expression in skin fibroblast cells<br>牛c-myc基因的克隆及其在皮肤成纤维细胞中的表达
%A Jiajia Xiao
%A Fengfeng Zhang
%A Xianrong Xiong
%A Yong Zhang
%A <br>肖佳佳
%A 张凤凤
%A 熊显荣
%A 张涌
%J 生物工程学报
%D 2011
%I 
%X In order to construct a eukaryotic expression vector of bovine c-myc gene, the coding sequence (CDS) of c-myc gene was amplified from bovine primordial genital ridges by RT-PCR. The CDS was subcloned into pMD19-T vector, and then inserted into vector pIRES2-AcGFP1-Nuc. After confirmed by restriction enzyme digestion and sequencing, the recombined plasmid was transfected into skin fibroblast cells. RT-PCR and Western Blotting were used to detect the expression of c-myc mRNA and protein, respectively. The results show that the complete CDS of c-myc gene was cloned from fetal bovine primordial genital ridges. The eukaryotic expression vector of bovine c-myc gene was constructed and efficiently expressed in the skin fibroblast cells. The present study will lay a good foundation for further study of c-myc gene function and bovine induced pluripotent stem cells from somatic cells by defined factors.
%K bovine
%K c-myc gene
%K iPS cells (induced pluripotent stem cells)
%K eukaryotic expression<br>牛，c-myc基因，iPS细胞，真核表达
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=9880CD65C317F63D57DF9D1ADE50ABE2&yid=9377ED8094509821&vid=DB817633AA4F79B9&iid=B31275AF3241DB2D&sid=BA5B7FED4FC3CDB1&eid=20154CF366EC08B1&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=0