%0 Journal Article
%T Genome shuffling method of Bacillus subtilis<br>枯草芽胞杆菌基因组混组方法
%A Junjie Yang
%A Wenchao Fan
%A Han Xiao
%A Chunhong Guan
%A Chuanzeng Cao
%A Haifeng Shao
%A Weihong Jiang
%A Sheng Yang
%A <br>杨俊杰
%A 范文超
%A 肖晗
%A 关春鸿
%A 曹传增
%A 邵海峰
%A 姜卫红
%A 杨晟
%J 生物工程学报
%D 2010
%I 
%X Genome shuffling methods were explored for Bacillus subtilis strain molecular breeding. Recycling protoplast fusion, recycling transformation and recycling universal transduction were used for genome shuffling in B. subtilis. Four strains with different nutrition-deficiency markers were used as initial strains. After five rounds protoplast fusion, transformation or transduction, the descendant with 4 markers had not been detected, and the rate of descendant with 3 markers achieved 4.53×10?4, 1.64×10?4, 4.47×10?3, respectively. A computer program was made to simulate the recycling fusion process. Base on simulation result and comparing the genome result of B. subtilis in this experiment and that of Streptomyces coelicolor reported in references, effective genome shuffling needs a high recombination rate of at least between 10?3 and 10?2.
%K genome shuffling
%K Bacillus subtilis
%K molecular breeding
%K protoplast fusion
%K transformation
%K transduction
%K in silico simulation<br>基因组混组，枯草芽胞杆菌，分子育种，原生质体融合，转化，转导，计算机模拟
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=3B3B27AA34443900A14085E3B99D4A00&yid=140ECF96957D60B2&vid=96C778EE049EE47D&iid=F3090AE9B60B7ED1&sid=09D89DB7E3DF529E&eid=FEF59218C1413604&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=0