%0 Journal Article
%T Synthesis and Identification of Penicillic-Acid Antigens from Penicillium cyclopium<br>圆弧青霉菌毒素-青霉酸人工抗原的合成与鉴定
%A Hongyu Lei
%A Hui Yuan
%A Jing Wu
%A Liyun Yuan
%A Lixin Wen
%A Hengjia Ni
%A <br>雷红宇
%A 袁慧
%A 邬静
%A 袁莉芸
%A 文利新
%A 倪姮佳
%J 生物工程学报
%D 2008
%I 
%X To establish a new immune assay for Penicillic Acid (PA) from Penicillium cyclopium, we studied the synthesis of conjugated complete antigens for penicillic acid. PA was conjugated to bovine serum album (BSA) and ovalbumin (OVA) by 1-ethyl-3-(3-dimethyl-aminopropyl) carbodiimide hydrochloride (EDC). The artificial antigens PA-BSA and PA-OVA were identified by ultraviolet spectrometric scanning, SDS-PAGE and immunization. Results showed that the absorption peak of conjugation were different from that of the carrier protein alone and of the PA. The conjugated ratio of PA and BSA was 23.2:1 and that of PA and OVA was 10.4:1. Balb/c mice were immunized by the artificial antigen of PA-BSA, with PA-OVA as coating antigen. The average titer of antiserums was more than 12 800 by indirect ELISA. The obtained antigens offered a basis for developing immunoassay method.
%K penicillic acid (PA)
%K artificial antigen
%K synthesis
%K ELISA<br>青霉酸
%K 人工抗原
%K 合成
%K ELISA
%K 圆弧
%K 霉菌毒素
%K 青霉酸
%K 人工抗原合成
%K Penicillium
%K Antigens
%K Identification
%K 免疫检测方法
%K 抗血清
%K ELISA
%K 包被抗原
%K 小鼠
%K 抗原免疫
%K 偶联比
%K 吸收峰
%K 抗原特征
%K 显示
%K 结果
%K 免疫试验
%K 动物
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=B935193B83EDE5B01E8893B2A510556B&yid=67289AFF6305E306&vid=B91E8C6D6FE990DB&iid=94C357A881DFC066&sid=9124D83E61CF1CD0&eid=F52FA2254444BE97&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=17