%0 Journal Article
%T Research of Real-time Fluorescent PCR in the Rapid Differential Detection of H5、 H9、 H7 Subtype Avian Influenza Inactivated Vaccines<br>实时荧光PCR技术快速鉴别检测H5、H9、H7亚型禽流感灭活疫苗的研究
%A HAN Jian-Feng
%A NING Yi-Bao
%A SONG Li
%A YANG Cheng-Huai
%A <br>韩剑锋
%A 宁宜宝
%A 宋立
%A 杨承槐
%J 生物工程学报
%D 2007
%I 
%X Specific primers and TaqMan MGB probes were designed with Primer Express 2.0 software according to the conserved region of the H5,H9,H7 subtype AIV hemagglutinin gene to make research of real-time fluorescent one-step PCR in the differential detection of H5,H9,H7 subtype avian influenza inactivated vaccines.The result showed that the method was specific and reproducible.No cross-reaction was discovered with other avian disease vaccines.Real-time fluorescent PCR provided a specific,sensitive,rapid and convenient method for the subtype identification of avian influenza inactivated vaccines.
%K real-time fluorescent PCR
%K avian influenza inactivated vaccine
%K differential detection<br>实时荧光PCR
%K 禽流感灭活疫苗
%K 鉴别检测
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=893AE993A934CD09&yid=A732AF04DDA03BB3&vid=EA389574707BDED3&iid=94C357A881DFC066&sid=85A6AA3FF013E1BF&eid=158793AD8125C377&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=14