%0 Journal Article
%T Bioconversion of D-fructose to D-allose by novel isomerases<br>以D-果糖为原料利用新型异构酶转化生产D-阿洛糖
%A Wei Bai
%A Yueming Zhu
%A Yan Men
%A Xiaobo Li
%A Ken Izumori
%A Yuanxia Sun
%A <br>柏玮
%A 朱玥明
%A 门燕
%A 李晓波
%A 何森健
%A 孙媛霞
%J 生物工程学报
%D 2012
%I 
%X Rare sugar is a kind of important low-energy monosaccharide that is rarely found in nature and difficult to synthesize chemically. D-allose, a six-carbon aldose, is an important rare sugar with unique physiological functions. It is radical scavenging active and can inhibit cancer cell proliferation. To obtain D-allose, the microorganisms deriving D-psicose 3-epimerase (DPE) and L-rhamnose isomerase (L-RhI) have drawn intense attention. In this paper, DPE from Clostridium cellulolyticum H10 was cloned and expressed in Bacillus subtilis, and L-RhI from Bacillus subtilis 168 was cloned and expressed in Escherichia coli BL21 (DE3). The obtained crude DPE and L-RhI were then purified through a HisTrap HP affinity chromatography column and an anion-exchange chromatography column. The purified DPE and L-RhI were employed for the production of rare sugars at last, in which DPE catalyzed D-fructose into D-psicose while L-RhI converted D-psicose into D-allose. The conversion of D-fructose into D-psicose by DPE was 27.34%, and the conversion of D-psicose into D-allose was 34.64%.
%K D-fructose
%K D-psicose
%K D-allose
%K bioconversion
%K D-psicose 3-epimerase
%K L-rhamnose isomerase<br>D-果糖
%K D-阿洛酮糖
%K D-阿洛糖
%K 生物转化
%K D-阿洛酮糖-3-差向异构酶
%K L-鼠李糖异构酶
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=878F493A8FA2CF12F483B2BC53648FB4&yid=99E9153A83D4CB11&vid=D3E34374A0D77D7F&iid=E158A972A605785F&sid=D0E8F9CBDBE0070C&eid=BC88D6B0750E09D1&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=0