%0 Journal Article
%T Enhancing expression efficiency of alkaline polygalacturonate lyase by constant cell concentration culture of the recombinant Pichia pastoris<br>恒细胞密度发酵策略提高重组毕赤酵母生产碱性果胶酶的表达效率
%A Huilin Wang
%A Jianghua Li
%A Long Liu
%A Jiangning Song
%A Guocheng Du
%A <br>王辉林
%A 李江华
%A 刘龙
%A 宋江宁
%A 堵国成
%J 生物工程学报
%D 2012
%I 
%X In order to enhance the alkaline polygalacturonate lyase (PGL) productivity by Pichia pastoris, we developed a constant cell concentration culture strategy by methanol feeding (called as CCCM culture) used in the continuous cultures. We controlled reasonable cell concentrations in the bioprocess by different strategies of methanol feeding. Using this CCCM culture with DCW 75 g/L, we significantly enhanced the PGL productivity (QV) and the average specific enzyme production rate (QX) of PGL to 6.11 U/(mL·h) and 81.5 U/(g·h), increased by 42.1% and 191.2% than the fed-batch culture with high cell density, respectively. The final PGL activity was 441.9 U/mL. Moreover, the extracellular protease concentration is 1.9 mg/L and the cell viability is more than 94% after 120 hour induction. The results show that this new strategy is advantageous in reducing proteolytic degradation and enhancing cell viability.
%K recombinant Pichia pastoris
%K cell concentration
%K alkaline polygalacturonate lyase (PGL)
%K fed-batch culture
%K CCCM culture
%K production intensity<br>重组毕赤酵母
%K 细胞密度
%K 碱性果胶酶(PGL)
%K 分批补料培养
%K 恒细胞密度发酵
%K 生产强度
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=8A6EC9640C158E7BB5949DA97B5BDED7&yid=99E9153A83D4CB11&vid=D3E34374A0D77D7F&iid=5D311CA918CA9A03&sid=AB8B0EE7E1A96CB2&eid=BFB3B49B74E638B4&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=0