%0 Journal Article
%T Analysis of interaction between insulin and its receptor by biofunctional chromatography<br>生物功能化色谱技术研究胰岛素与其受体间的相互作用
%A Wen M
%A Qiong Guo
%A Feng Qu
%A Qibin Huang
%A Yulin Deng
%A <br>马 文
%A 郭 琼
%A 屈 锋
%A 黄启斌
%A 邓玉林
%J 生物工程学报
%D 2008
%I 
%X We used biofunctional chromatography to study the interaction between insulin and immobilized insulin receptor(IR). The heterogeneous insulin receptor extract from Wistar mouse liver was immobilized onto the surface of an immobilized artificial membrane through coating. The detergent was removed by dialysis to reduce its interference with the chromatography. To optimize the protein immobilization, we optimized the coating conditions at: pH 7.2, ion strength 20 mmol, and organic solvent 2% (v/v). The competition and self-displacement interacting study of insulin with IR was conducted by the zonal elution using insulin as the self-competition agent, the result indicated that the insulin receptor was active and interacted with insulin. In addition, relative binding constant was determined by frontal analysis by changing the concentration of insulin in the mobile phase.
%K biofunctional chromatography
%K immobilized artificial membrane
%K protein-protein interaction
%K zonal elution
%K frontal analysis
%K binding constants<br>生物功能化色谱
%K 固定化人工膜
%K 蛋白质相互作用
%K 区带洗脱
%K 前沿分析
%K 结合常数
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=659C02E0E010DED68F7E218A574EDC68&yid=67289AFF6305E306&vid=B91E8C6D6FE990DB&iid=CA4FD0336C81A37A&sid=06EA2770E96C5402&eid=7E8E8B150580E4AB&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=13