%0 Journal Article
%T Application of Spindle-view in the Enucleation Porcine of Oocytes<br>Spindle-view系统在猪卵母细胞去核中的应用
%A YANG Yu
%A DAi Jian-Jun
%A ZHANG Ting-Yu
%A WU Hua-Li
%A CHEN Xue-Jin
%A ZHANG De-Fu
%A MA Heng-Dong
%A <br>杨宇
%A 戴建军
%A 张廷宇
%A 吴华莉
%A 陈学进
%A 张德福
%A 马恒东
%J 生物工程学报
%D 2007
%I 
%X The Spindle-view, a specialized instrument for observing spindle image, was applied to observe the meiotic spindles of vitro matured porcine oocytes at 36, 42, 44, 48h, and enucleation from porcine, comparing to the previously methods (McGrath-Solter's method and two-step-squeezing method) in the enucleated. The results showed that: (1) there was no noticeable differences at vicinity of spindle images and 1st polar body among in vitro matured porcine oocytes at 40-48 h under the instrument; (2) Spindle-view is suitable for the observation of meiotic spindles of matured oocytes and enucleation from porcine; the modified Spindle-view method for enucleation is significantly better than McGrath-Solter' s method and two-step-squeezing method in the enucleated rates (95.5%, 42.1%, 74.2%, P < 0.0l) of absolutely removing nuclei matter; (3) the spindle images could be used to monitor the oocyte qualities.
%K Spindle-view<br>猪卵母细胞
%K 去核
%K 减数分裂纺锤体
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=D641C0FE5BF2DB7EBF8128E5FC9F2D66&yid=A732AF04DDA03BB3&vid=EA389574707BDED3&iid=B31275AF3241DB2D&sid=D932AD0F8FDA3032&eid=F9AB8F3D624A89DB&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=15