%0 Journal Article
%T Priming with an HEV Th Epitope Can Improve the Humoral Immunogenicity of Its Native Protein<br>戊肝病毒Th表位肽免疫可增强其载体蛋白的体液免疫应答
%A LIN Chun-Xin
%A WU Ting
%A WU Xiao-Lu
%A XIE Ming-Hui
%A CHENG Tong
%A LI Shao-Wei
%A ZHANG Jun
%A XIA Ning-Shao
%A <br>林春鑫
%A 吴婷
%A 伍小路
%A 谢明辉
%A 程通
%A 李少伟
%A 张军
%A 夏宁邵
%J 生物工程学报
%D 2007
%I 
%X A dominant H-2d restricted Th epitope P34 was found to be contained in recombinant particulate hepatitis E virus (HEV) vaccine HEV 239. In this paper, the cellular immune response induced in P34 immunized BALB/c mice were studied and the priming effect of P34 was characterized. Groups of BALB/c mice were subcutaneously (s. c.) immunized with P34, splenocytes were then stimulated with P34 and HEV 239 protein, cellular immune response was assayed by IFN-gamma-ELISPOT, flow cytometry and T cell proliferation experiments. Results showed that P34 immunized BALB/c splenocytes responsed to P34 and HEV 239 protein stimulation in IFN-gamma-ELISPOT, flow cytometry and T cell proliferation experiments. After depletion of the CD4+ T cells from the immunized splenocytes by magnetic separation, the response decreased to the background level while almost no influence was observed after CD8 + T cells depletion which showed that the cells responsible for IFN-gamma secretion were mainly CD4+ T cells. Then mice were primed with P34 and boosted with its vector protein, E2, the E2 specific antibody titer were assayed. Results showed that after P34 priming, some of the 10 microg, 20 microg E2 boosted mice could develop anti-E2 antibody 1 week later and all the mice had detectable antibody 3 weeks after boosting. In the control peptide P18 priming group, even after boosting with 20 microg E2, anti-E2 antibody couldn't be detected until the end of this experiment. The results showed that priming with P34 epitope could increase the immunogenicity of its vector protein, E2, in BALB/c mice.
%K Hepatitis E virus
%K Th cell
%K T epitope
%K immunogenicity<br>戊型肝炎病毒
%K T辅助细胞
%K T细胞表位
%K 免疫原性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=59251FF87D2E0875&yid=A732AF04DDA03BB3&vid=EA389574707BDED3&iid=0B39A22176CE99FB&sid=85002451B65CE0D1&eid=AA5FB09E1F81059E&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=15