%0 Journal Article
%T Molecular Cloning and Characteristics of cDNA Encoding Pig β6 Subunit for FMDV Receptor<br>口蹄疫病毒受体猪源β6亚基基因的克隆和分子特征
%A GAO Shan-Dian
%A DU Jun-Zheng
%A CHANG Hui-Yun
%A CONG Guo-Zheng
%A SHAO Jun-Jun
%A YI Hua-Shan
%A ZHOU Jian-Hua
%A XIE Qing-Ge
%A <br>高闪电
%A 独军政
%A 常惠芸
%A 丛国正
%A 邵军军
%A 易华山
%A 周建华
%A 谢庆阁
%J 生物工程学报
%D 2007
%I 
%X In order to study the roles of integrin beta6 in Foot-and-Mouth Disease Virus infection, pig integrin beta6 was firstly molecularly cloned from RNA of the tongue and lung of recovered pig infected experimentally with foot-and-mouth-disease virus (FMDV), and was compared with the beta6 gene of other animals available in GenBank at nucleotide and amino acid leves. GeneBank association number of the beta6 gene is EF432729. Pig integrin beta6 gene (2367bp) encodes a polypeptide of 788 amino acids consisting of 9 potential N-linked glycosylation sites, 3 Glycosaminoglycan attachment sites, a cGMP-dependent protein kinase phosphorylation site, 10 Protein kinase C phosphorylation sites, 2 EGF-like domains and 2 cysteine-rich regions. Pig integrin beta6 subunit has a 26-residue putative signal peptide, a 681-residue ectodomain, a 29-residue transmembrane domain, and a 52-residue cytoplasmic domain. 11 mutant nucleotides were found in beta6 gene coding region and 9 amino acids were changed. The nucleotide sequence similarity of integrin beta6 gene between rheses monkey, mouse, Norway rat, dog, guinea pig, human, bovine, sheep is 79.5%, 84.9%, 85.4%, 85.2%, 88.7%, 90.1%, 91.9% and 91.9%, and the amino acid sequence similarity is 93.5%, 88.2%, 88.5%, 88.3%, 91.0%, 92.8%, 93.3% and 93.4% respectively. This study will lay a foundation for understanding the interactions of FMDV with receptors.
%K 口蹄疫病毒
%K 受体
%K 猪整联蛋白β6基因
%K 分子特征
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=AB638E13AEFF58F1&yid=A732AF04DDA03BB3&vid=EA389574707BDED3&iid=94C357A881DFC066&sid=9C6D270E246D943F&eid=0FBB0D015A3E9A88&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=18