%0 Journal Article
%T Chemical Induction--New Strategy of Oocyte Enucleation<br>化学诱导法——卵母细胞去核新策略
%A WANG Qiang
%A GU Ling
%A ZHANG Yong
%A <br>王强
%A 顾玲
%A 张涌
%J 生物工程学报
%D 2003
%I 
%X Mammalian somatic cloning has got great progress during past few years,however,the efficiency of nuclear trasfer remains low.In order to improve this technique,different perspectives of which are studying.Accordingly,the method of oocyte enucleation also becomes a focus.But the physical enucleation is technically demanding, time-consuming, inherently invasive and clearly damaging to cytoplast spatial organization. As a alternative strategy to traditional method, Demecolcine-induced enucleation(IE) attracted the scientist's eyes. Nuclear transfer procedure assited with IE,factors releated with success rate of IE and effects of IE on the oocytes and cloned embryos are mainly discussed in this review. At the same time ,combining with author's research, the existing problems of IE and potential improved measures of some key steps in IE procedure are also elucidated. However, the utility of the demecolcine-induced enucleation protocol will require further deep investigation.
%K induced-enucleation(IE)
%K demecolcine
%K nuclear transfer
%K cloning efficiency<br>诱导去核
%K 脱羰秋水仙碱(Demecolcine)
%K 核移植
%K 克隆效率
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=1DCF74B65609A93A&yid=D43C4A19B2EE3C0A&vid=2A8D03AD8076A2E3&iid=B31275AF3241DB2D&sid=50FF665B2730AEEC&eid=34A7AB0452E6AF23&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=2&reference_num=30