%0 Journal Article
%T Construction of Recombinant Fowlpox Virus Coexpressing gp120 of Chinese HIV-1 Strain and IL-18 and Its Immunogenicity in Mice<br>共表达HIV-1中国流行株gp120与IL-18重组鸡痘病毒的构建及其免疫原性观察
%A Wen-Zheng Jiang
%A Ning-Yi Jin
%A Zi-Jian Li
%A Li-Shu Zhang
%A Wen-Yu Han
%A <br>江文正
%A 金宁一
%A 李子健
%A 张立树
%A 韩文瑜
%J 生物工程学报
%D 2004
%I 
%X To screening out Chinese vaccine candidate against HIV-1, Chinese vaccine strain 282E4 of fowlpox virus was used as the vector to construct the recombinant fowlpox virus (rFPV) coexpressing gp120 of Chinese HIV-1 strain and IL-18, and the recombinant virus was indentified by PCR and Western blot. The specific DNA fragment could be amplified by PCR from the genome of rFPV. Western blot analysis showed that gp120 and IL-18 could be expressed not only in chicken embryo fibroblast (CEF) cells infected by rFPV, but also in mammalian cells infected by rFPV. After the recombinant fowlpox virus was inoculated into BALB/c mice, the spleen specific CTL activities and serum antibodies in the immunized mice were detected, which demonstrated that the rFPV had good immunogenicity and could induce BALB/c mice to produce specific humoral and cellular immunity. IL-18 palyed the role of immunoadjuvant. The study lays the basis on the preparation of genetic engineering live vector vaccine against HIV-1.
%K HIV-1
%K gp120
%K IL-18
%K fowlpox virus
%K antibody
%K cytotoxicity T lymphocyte<br>HIV-1，gp120，白细胞介素18，鸡痘病毒，抗体，细胞毒T淋巴细胞
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=C2C06F5440AA43B2&yid=D0E58B75BFD8E51C&vid=A04140E723CB732E&iid=38B194292C032A66&sid=5FF9F4F7CB1800C7&eid=4B168891B5E5FB30&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=1&reference_num=8