%0 Journal Article
%T Expression and Analysis of Recombinant pIL-18 in Pichia pastoris<br>猪IL-18在毕赤酵母中的表达及活性分析
%A SONG Shi-Bin
%A JING Zhi-Zhong
%A CHEN Guo-Hu
%A WANG Xiao-Xiao
%A ZONG Rui-Qian
%A <br>宋世斌
%A 景志忠
%A 陈国华
%A 王笑笑
%A 宗瑞谦
%J 生物工程学报
%D 2007
%I 
%X The porcine IL-18 gene was amplified from recombinant plasmid pGEM-IL-18 by PCR,then the pPIC9K-IL-18 of fusion expression vector was constructed by inserting IL-18 fragment,and was transformed to GS115 by electroporation,multi-copy recombinant strains were screened by G418.The expression of recombinant fusion protein was induced by methanol,SDS-PAGE was used to analyze expression product,fusion protein was purified by Sephadex G-100 column,bioactivity of IL-18 was measured by MTT assays.Experiment results show fusion protein of pIL-18 secreted by GS115,expression reaches the secretion peak of 160mg/L at 72h.We have expressed and purified successfully the recombinant pIL-18 with obvious biological activity in Pichia pastoris.
%K pIL-18<br>毕赤酵母
%K 基因表达
%K 纯化
%K 生物活性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=57F3DA1508E6BF08&yid=A732AF04DDA03BB3&vid=EA389574707BDED3&iid=94C357A881DFC066&sid=F2947E14627CD734&eid=CE504F5B1E192581&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=14