%0 Journal Article
%T Infectious Bovine Rhinotracheitis Viral gG Expression and gG-ELISA Development<br>牛传染性鼻气管炎病毒gG蛋白的表达及gG-ELISA的建立
%A YAN Bang-Fen
%A CHEN Zeng
%A ZHANG Shu-Huan
%A LIN Xiang-Mei
%A CHEN Ying-Yu
%A CHAO Yan-Jie
%A LI De-Xue
%A SONG Nian-Hu
%A CHEN Huan-Chun
%A GUO Ai-Zhen
%A <br>颜邦芬
%A 陈锃
%A 张书环
%A 林祥梅
%A 陈颖钰
%A 晁彦杰
%A 李德学
%A 宋念华
%A 陈焕春
%A 郭爱珍
%J 生物工程学报
%D 2007
%I 
%X Taking the genome DNA of Infectious Bovine Rhinotracheitis Virus (IBRV) as the template, the gG gene was amplified with PCR and cloned into the T cloning vector pMD18-T. After being identified by restriction digestion and DNA sequencing, the insert was subcloned into the expression vector pGEX-KG. Sodium docecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and Western blot assay showed that this gene was expressed as both soluble form and inclusion body by the transformed E. coli BL21 strain (DE3). The fusion protein was purified and used as the coating antigen to develop the indirect Enzyme-Linked Immunosorbent Assay (ELISA). Comparison between this gG-ELISA and commercial IBRV gB-ELISA Kit (IDEXX) was made in the detection of 380 cow serum samples. The results demonstrated an agreement of 92%. By using this novel gG-ELISA, 1248 cow serum samples were tested and the average positive rate of IBRV antibodies for imported cows is 21.7%, while the positive rate ranged greatly from 0.0%-41.5% for Hubei local Chinese Black and White Dairy Cows.
%K ELISA<br>牛传染性鼻气管炎病毒
%K gG基因
%K 原核表达
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=6B92EE19794CE683&yid=A732AF04DDA03BB3&vid=EA389574707BDED3&iid=94C357A881DFC066&sid=EDAEFEDAC1DCCBA4&eid=A9C78B2A6AAEEAAD&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=4&reference_num=18