%0 Journal Article
%T Multiplex fluorescent real-time PCR detection of bovine, goat and sheep derived materials in animal products<br>多重实时荧光PCR检测牛、山羊和绵羊源性成分
%A Shaoling Zeng
%A Zhifeng Qin
%A Zhouxi Ruan
%A Qunyi Hua
%A Tikang Lü
%A Jianqiang Lu
%A Shukun Chen
%A Chenfu Cao
%A Caihong Zhang
%A Jie Sun
%A Bing Chen
%A Shaojing Wu
%A <br>曾少灵
%A 秦智锋
%A 阮周曦
%A 花群义
%A 卢体康
%A 吕建强
%A 陈书琨
%A 曹琛福
%A 张彩虹
%A 孙洁
%A 陈兵
%A 吴绍精
%J 生物工程学报
%D 2009
%I 
%X We designed the specific primers and TaqMan probes targeting cytochrome b genes of mitochondrial DNA from bovine, goat and sheep. We used different fluorescents to label the probes. After optimization of reaction conditions, we set up a multiplex fluorescent real-time PCR method to detect bovine, goat and sheep derived materials, simultaneously. We finished the detection tests of 17 kinds of animal DNA and 200 DNA samples from different sources with the developed method and the National Standard GB/T 20190Y-2006 routine PCR method. The coincidence rate of these two methods was 100%. Without electrophoresis or restriction digestion, the developed method could reduce the test time to one third as routine PCR and identify three kinds of animal derived materials including bovine, goat and sheep in one reaction. The developed method was approximately 10 times more sensitive than routine PCR, and was applicable to identifications of bovine, goat and sheep derived materials in feed stuff, meat, milk, pelt and grease, etc. The study showed that the developed real-time PCR method is a rapid, sensitive and efficacious detection assay for bovine, goat and sheep derived materials in animal products.
%K cytochrome b gene
%K multiplex fluorescent real-time PCR
%K bovine
%K goat and sheep derived materials<br>细胞色素b基因
%K 多重实时荧光PCR
%K 牛、山羊和绵羊源性成分
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=23F819EC39D4B9655F7F7268BA4B514B&yid=DE12191FBD62783C&vid=C5154311167311FE&iid=CA4FD0336C81A37A&sid=67A4C102D80D32DF&eid=63F6C8363E1A77A6&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=1&reference_num=17