%0 Journal Article
%T Expression and Purification of Heptad Repeat Region of the Mumps Virus F Protein and Analysis of Characteristics<br>腮腺炎病毒F蛋白七肽重复序列的表达、纯化及特性分析
%A LIU Yue-Yong
%A FENG Ming-Guang
%A ZHU Jie-Qing
%A JIANG Li-Jie
%A Tien Po
%A {}
%A <br>刘月永
%A 冯明光
%A 朱杰青
%A 姜立杰
%A 田波
%J 生物工程学报
%D 2004
%I 
%X Two Heptad repeat motifs (HR1 and HR2) from paramyxoviruses F protein could form thermostable heterodimers containing high alpha-helix while virus infected host cell. Following that the viral membrane and the host cell membrane were juxtaposed, which leads to membrane fusion. Mumps virus (MuV) is a member of the genus Rubulavirus in the family of Paramyxoviridae. MuV could use similar infection mechanism as well as other paramyxoviruses. In this study the HR1 and HR2 regions of MuV F protein were predicted by a computer program and expressed in E. coli with the GST fusion expression system. The GST fusion or GST-removed proteins were purified with Gluthathion Sepharose 4B Column. GST pull-down experiment suggested the interaction of HR1 and HR2 peptides, and analysis of gel filtration showed two peptides could form multimer, which indicates that the HR regions of MuV F protein may play an important role in virus fusion.
%K Mumps virus (MuV)
%K F protein
%K HR1
%K HR2
%K GST pull-down<br>腮腺炎病毒（Mumps
%K virus
%K MuV），F
%K 蛋白，七肽重复序列(HR)，GST
%K pull-down
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=538126096288696E&yid=D0E58B75BFD8E51C&vid=A04140E723CB732E&iid=38B194292C032A66&sid=389106FB36CA8332&eid=B9B90065CF5CD7F0&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=21