%0 Journal Article
%T Expression of Human LNα4LG4-5 in Pichia Yeast<br>人LNα4LG4-5在毕赤酵母中的表达研究
%A LIAN Ji-Qin
%A DAI Xu-Fang
%A LI Xiao-Hui
%A HE Feng-Tian
%A <br>连继勤
%A 戴旭芳
%A 李晓辉
%A 何凤田
%J 生物工程学报
%D 2006
%I 
%X To carry out the secretive expression of human laminin alpha4 chain LG4-5 module (hLNalpha4LG4-5), recombinant expression plasmid pPICZalphaA-LG45 was constructed by inserting of hLNalpha4LG4-5 cDNA into yeast expression vector pPICZaA. The hLNalpha4LG4-5 protein was expressed in GS115 Pichia yeast strain after induced by methanol, and purified target protein can obviously promote the expansion and adhesion of 293 cells.
%K Pichia yeast<br>hLNα4LG4-5
%K Pichia
%K yeast
%K 基因表达
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=E1EA7E53ABA5D56F&yid=37904DC365DD7266&vid=BC12EA701C895178&iid=0B39A22176CE99FB&sid=6ED15D8DCB279BC4&eid=D6354F61445E9456&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=11