%0 Journal Article
%T Expression of snake venom thrombin-like enzyme calobin in Pichia pastoris<br>蛇毒类凝血酶calobin在毕赤酵母中的表达
%A Shengling Yuan
%A Peng Wang
%A Haoxia Tao
%A Dewen Zhan
%A Yanchun Wang
%A Lingchun Wang
%A Chunjie Liu
%A Zhaoshan Zhang
%A <br>袁盛凌
%A 王芃
%A 陶好霞
%A 展德文
%A 王艳春
%A 王令春
%A 刘纯杰
%A 张兆山
%J 生物工程学报
%D 2009
%I 
%X Thrombin-like enzymes (TLEs) are studied widely because of their therapeutic potential in myocardial infarction and thrombotic diseases. We synthesized the DNA fragment encoding thrombin-like enzyme calobin from Agkistrodon caliginosus (Korean Viper) venom by fusion PCR and expressed it in Pichia pastoris. After induction by 0.5% methanol for 48 h,the expression level of recombinant calobin reached 3.5 g/L in medium. The recombinant calobin was purified by Q-Sepharose Fast Flow ion-exchange chromatography a...
%K thrombin-like enzyme
%K Pichia pastoris
%K calobin
%K fibrinogen<br>蛇毒类凝血酶
%K 毕赤酵母
%K calobin
%K 纤维蛋白原
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=90C27C3FBF5F8BF13A2FAA9009E07B62&yid=DE12191FBD62783C&vid=C5154311167311FE&iid=E158A972A605785F&sid=E3D3D8D1B650AE1E&eid=640CCB6E396307A8&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=1&reference_num=15