%0 Journal Article
%T Effect of trehalose on the freeze-dried boar spermatozoa<br>海藻糖对猪精子冷冻真空干燥保存效果的影响
%A Xiangqian Meng
%A Xiaolong Gu
%A Caifeng Wu
%A Jianjun Dai
%A Tingyu Zhang
%A Yini Xie
%A Zhiqiang Wu
%A Liang Liu
%A Hengdong Ma
%A Defu Zhang
%A <br>孟祥黔
%A 顾晓龙
%A 吴彩凤
%A 戴建军
%A 张廷宇
%A 谢一妮
%A 吴志强
%A 刘亮
%A 马恒东
%A 张德福
%J 生物工程学报
%D 2010
%I 
%X After freeze-drying, the ultrastructure of boar sperms was observed by optical and electron microscopy. The in vitro development ability of the sperm was also examined with intracytoplasmic sperm injection (ICSI). The rate of male pronuclear formation was (68.52%), for cleavage (59.17%) and for blastocyst formation (19.16%) of the trehalose group (0.2 mol/L), significantly higher than those of the 50 mmol/L EDTA group (64.59%, 56.26% and 15.62%) and the control group (35.36%, 52.33% and 8.60%) (P<0.05). After storage for 60, 120 and 180 d at 4°C, no significant difference in the in vitro development was observed (P>0.05). The male pronuclear, cleavage and blastocyst formation after ICSI with freeze-dried spermatozoa incubated for 1 h was superior than those incubated for 2 h (P<0.05). No significant differences in the structures after stored at 4°C or ?20°C (P>0.05) were observed between the trehalose group and EDTA group. The percent of B grade freeze-dried boar spermatozoa in the trehalose group was higher than that of the EDTA group (P<0.05). Based on the ultrastructure observation, main cryogenic damage in freeze-dried boar spermatozoa was swelling, damage or rupture in the sperm acrosome, neck and tail.
%K boar spermatozoa
%K freeze-drying
%K intracytoplasmic sperm injection<br>猪精子，冷冻真空干燥，单精子注射
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=0E49F5EF4A0CEA2D2F745CD8AE016D5F&yid=140ECF96957D60B2&vid=96C778EE049EE47D&iid=5D311CA918CA9A03&sid=CF89D3C02791C82E&eid=1529CB0C2A6A7E90&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=0