%0 Journal Article
%T Recycle of spent cells from anaerobic succinate fermentation<br>发酵产丁二酸过程中废弃细胞的循环利用
%A Xuefei Bai
%A Kequan Chen
%A Guizi Ye
%A Xiumei Huang
%A Jian Li
%A Min Jiang
%A <br>白雪飞
%A 陈可泉
%A 叶贵子
%A 黄秀梅
%A 李建
%A 姜岷
%J 生物工程学报
%D 2010
%I 
%X Spent cells recovered from anaerobic fermentation by Actinobacillus succinogenes were used as nitrogen source for succinic acid production. Three methods were investigated for cell wall-breaking. The results showed that enzymatic hydrolysis was more effective for higher succinic acid yield. When the enzymatic hydrolysate of spent cells was added to reach a total nitrogen concentration 1.11 g/L (equivalent to 10 g/L yeast extract), the succinic acid concentration was 42.0 g/L, but it increased slightly when enhancing the level of enzymatic hydrolysate. However, when 5 g/L yeast extract was supplemented with the enzymatic hydrolysate of spent cells, the succinic acid concentration reached 75.5 g/L after 36 hours and, the succinic acid productivity was 2.10 g/(L·h), which increased by 66.7% compared with the fermentation using 10 g/L yeast extract. Therefore, enzymatic hydrolysate of spent cells could replace 50% yeast extract in the original medium for succinic acid production.
%K recycle spent cells
%K enzymatic hydrolysis
%K nitrogen source
%K succinic acid<br>废弃细胞，酶水解，氮源，丁二酸
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=C736E3A1CB2244E8882763187799F01E&yid=140ECF96957D60B2&vid=96C778EE049EE47D&iid=9CF7A0430CBB2DFD&sid=C700F38C49C581D7&eid=B9CA56463DAD3251&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=0