%0 Journal Article
%T Development of one step RT-PCR technique for detection of H7 subtype avian influenza<br>H7亚型禽流感病毒一步法RT-PCR检测方法的建立
%A Hongmei Bao
%A Xiurong Wang
%A Qimeng Tao
%A Dongdong Cai
%A Fumei Wang
%A Hualan Chen
%A <br>包红梅
%A 王秀荣
%A 陶启蒙
%A 蔡东东
%A 王馥梅
%A 陈化兰
%J 生物工程学报
%D 2009
%I 
%X According to 45 hemagglutinin(HA) gene sequences of H7 subtype of avian influenza virus(AIV),a pair of specific oligonucleotide primers was designed.We developed one step RT-PCR for detecting AIV subtype H7.Sensitivity to detection of allantoic fluid by one step RT-PCR reached 105.5 EID50/mL and detection of swab samples reached 103 EID50/mL.We simultaneity detected the tissue and swab samples infected with H7 subtypes AIV by one step RT-PCR and virus isolation method.The results showed that the sensitivity...
%K avian influenza virus
%K RT-PCR
%K H7N1
%K subtype differentiation<br>禽流感病毒
%K 反转录聚合酶链反应
%K H7N1
%K 亚型鉴别
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=0A4B1D4B18BC068A063D4486ED03BC1E&yid=DE12191FBD62783C&vid=C5154311167311FE&iid=708DD6B15D2464E8&sid=B221F15A486F30C1&eid=8D0822DF60ACC08F&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=1&reference_num=20