%0 Journal Article
%T Functional Expression of DNA Binding Domain of Zif268 in Escherichia coli<br>Zif268的锌指DNA结合区在大肠杆菌中的功能性表达
%A ZHAO Zhi-Hu XU Quan-Bin MA Qing-Jun
%A <br>赵志虎
%A 胥全彬
%A 马清钧
%J 生物工程学报
%D 2004
%I 
%X As the ubiquitous nucleic acids recognizing motif, Zinc finger protein play important role in regulation of gene expression. The study of recognization specific will greatly facilitate understanding the delicate interaction of Zinc finger protein and DNA. By the choice of expression vector, the induction and culture conditions, the DNA binding domain of Zif268 was expressed in Escherichia coli partly solubly. The gel mobility shift assay shows that purified DNA binding domain can bind its natural target sequence specifically, which indicates the DNA binding domain remains its DNA binding activity in Escherichia coli. The functional expression of DNA binding domain of Zif268 will greatly facilitate the development of in vivo genetic selection assay for the study of Zinc fingers-DNA interaction.
%K Zinc-finger protein
%K Soluble expression
%K Gel mobility shift assay<br>锌指蛋白，
%K 可溶性表达，
%K 凝胶迁移率移动试验
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=0E56D196426C6FA4&yid=D0E58B75BFD8E51C&vid=A04140E723CB732E&iid=38B194292C032A66&sid=EA64BD0B2FF6E786&eid=26AEEED215BE97D0&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=13