%0 Journal Article
%T Oriented Immobilization of Human IgG by Protein A on Imaging Ellipsometry Biosensor<br>A蛋白定向固定抗体用于椭偏光学生物传感器免疫检测
%A MENG Yan-Li
%A WANG Zhan-Hui
%A JIN Gang
%A <br>孟艳丽
%A 王战会
%A 靳刚
%J 生物工程学报
%D 2004
%I 
%X The biosensor based on optical imaging ellipsometry, can be used to detect directly, without labeling, the surface concentration of biomolecules on solid surface. The feasibility of using protein A to immobilize antibody on the silicon surface of the imaging ellipsometry biosensor was investigated in this study. The results showed that the anti-IgG immobilized by the protein A on silicon surface could bind effectively human IgG, and the human IgG immobilized on silicon surface by protein A bound more polyclonal antibody molecules than that immobilized on silicon surface directly, suggesting that protein A might block the surface to prevent the absorption of human IgG on surface directly, which might compromise its native configuration. The silicon surface modified with protein A is expected to be used to immobilize a variety of antibodies, as protein A can bind selectively the Fc regions of many mammalian IgG. The combination of imaging ellipsometry and the protein A surface modification has the potential to be developed into immunoassays of high sensitivity.
%K protein A
%K imaging ellipsometry biosensor
%K label-free immunoassay<br>A蛋白，椭偏光学生物传感器，无标记免疫检测技术
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=CA43021334603DFC&yid=D0E58B75BFD8E51C&vid=A04140E723CB732E&iid=CA4FD0336C81A37A&sid=480C51B1F0CE0AB6&eid=DDD31293A7C7D057&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=9&reference_num=9