%0 Journal Article
%T Studies of the Expression, Purification, Renaturation and Biologic Activity of an Anti-CEA Immunotoxin<br>抗CEA免疫毒素的表达、纯化、复性与生物学活性的研究
%A YANG Hui
%A HE Dan
%A CHAO Kai
%A LIN Qing
%A YOU Song
%A HUANG Hua-Liang
%A <br>杨慧
%A 何丹
%A 晁开
%A 林晴
%A 游松
%A 黄华樑
%J 生物工程学报
%D 2004
%I 
%X A recombinant immunotoxin named CEA/PE38/KDEL was constructed, which was composed of anti-CEA single-chain Fv and the truncated and modified form of Pseudomonas exotoxin (PE38/KDEL). The CEA/PE38/KDEL immunotoxin was expressed in the E.coli strain BL21(DE3)-star as inclusion bodies. The denatured inclusion bodies were purified with Ni-NTA chelate agarose, then the constant gradient dialysis was used to perform the refolding of the CEA/PE38/KDEL immunotoxin. Results of FACS and MTT assay indicate that the refolded immunotoxins keep potent and specific cytotoxicity to tumor cells bearing CEA antigens.
%K CEA
%K recombinant immunotoxin
%K affinity chromatography
%K protein renaturation
%K cytotoxicity<br>CEA，重组免疫毒素，亲和层析，蛋白复性，体外杀伤活性
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=D0F441882F2E7F8A&yid=D0E58B75BFD8E51C&vid=A04140E723CB732E&iid=38B194292C032A66&sid=375BEEEA164CFE59&eid=381FB4265090A8E0&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=12