%0 Journal Article
%T Synergistic effect of feruloyl esterase and cellulase in hydrolyzation of steam-exploded rice straw
阿魏酸酯酶和纤维素酶在水解汽爆稻草中的协同作用
%A Wei Zeng
%A Hongzhang Chen
%A
曾薇
%A 陈洪章
%J 生物工程学报
%D 2009
%I
%X Ferulic acid esterase (FAE) was used to hydrolyze feruloyl ester linkages between hemicellulose and lignin in natural lignocellulose, and the possibility of FAE accelerating cellulase to hydrolyze steam-exploded rice straw by breaking covalent linkages was studied. When the dosage of FAE was 240 mu/g substrate, the cellulose conversion rate and the weight-loss rate of insoluble substrate at 72 h were respectively 32.00% and 32.77%, more than without using FAE; Cellulose conversion rate and the weight-loss rate of insoluble substrate were respectively 29.85% and 32.48% with FAE (300 mu/g substrate) processing time of 120 min. By comparison of the accessibility and FT-IR spectra of lignocellulosic material treated by different enzyme methods, it indicated that FAE hydrolyzed some ester bonds within it, and improved the accessibility by over 50%. It is concluded that FAE and cellulase have great synergistic effect, and FAE can help cellulase hydrolyze natural lignocellulose and enhance hydrolytic efficiency.
%K ferulic acid esterase
%K cellulose
%K steam-exploded rice straw
%K synergistic effect
阿魏酸酯酶
%K 纤维素酶
%K 汽爆稻草
%K 协同作用
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=7D42DDFDA06BC962334ADC50EC20DEAD&yid=DE12191FBD62783C&vid=C5154311167311FE&iid=CA4FD0336C81A37A&sid=147F62B13FFBD5C1&eid=04011F6519DE775C&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=19