%0 Journal Article
%T Change of Hydrolase Activity in Germinating Seeds of trxS Transgenic Barley<br>转trxS基因大麦发芽种子水解酶活性的变化
%A Li Wei
%A Weiwei Kong
%A Jun Yin
%A <br>卫丽
%A 孔维威
%A 尹钧
%J 生物工程学报
%D 2008
%I 
%X Genetic modification of barley variety can be an efficient way to improve beer quality. The objective of this study was to understand the effect of trxS gene on hydrolases activities in transgenic and non-transgenic barley seeds. The results showed that a-amylase, free b-amylase and limit dextrinase activity were increased in transgenic seeds in comparison with non-transgenic seeds. Sulfhydryl content of protein in transgenic seeds was also higher than that in non-transgenic seeds, suggesting that trxS gene could express in barley seeds, which opens a new way for breeding new barley varieties to improve beer quality.
%K trxS gene
%K germination
%K a-amylase
%K b-amylase
%K limit dextrinase
%K sulfhydryl<br>trxS基因
%K 发芽
%K α-淀粉酶
%K β-淀粉酶
%K 极限糊精酶
%K 巯基
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=C8A350789A8533BB2388CA293BC60777&yid=67289AFF6305E306&vid=B91E8C6D6FE990DB&iid=9CF7A0430CBB2DFD&sid=28652329520A4AA7&eid=142CA2F7BF1B30ED&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=1&reference_num=22