%0 Journal Article
%T Technological Process of Cell Disruption for Extracting Astaxanthin from Phaffia rhodozyma by Acid Method under Autoclave Conditions<br>高温湿热酸法破壁提取法夫酵母胞内虾青素
%A Baoju Lu
%A Anfeng Xiao
%A Lijun Li
%A Hui Ni
%A Huinong Cai
%A Wenjin Su
%A <br>卢宝驹
%A 肖安风
%A 李利君
%A 倪辉
%A 蔡慧农
%A 苏文金
%J 生物工程学报
%D 2008
%I 
%X Phaffia rhodozyma is one of the organisms for production of astaxanthin, and the key process for extracting intracellular astaxanthin is cell disruption. In this work, cell disruption for extracting astaxanthin from Phaffia rhodozyma was studied with autoclave method at low acid concentration. The optimum disrupting conditions were: autoclave pressure 0.1 MPa, 121oC; hydrochloric acid concentration 0.5 mol/L; liquid to material ratio (V/W) 30 mL/g dry cell weight and disruption time 2 min. Under the optimum conditions, medium scale experiment showed that astaxanthin and total carotenoids recovery from Phaffia rhodozyma were (84.8 ± 3.2)% and (93.3 ± 2)%, respectively. This new method can lead to no poisonous residues and get high extraction yield, which have good prospects to be put into industrial production
%K Phaffia rhodozyma
%K cell disruption
%K astaxanthin extraction
%K autoclave
%K hydrochloric acid<br>法夫酵母
%K 细胞破壁
%K 虾青素提取
%K 高温湿热
%K 盐酸
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=95D38B3F4DF2A6D2A8EEE976B6B9B66D&yid=67289AFF6305E306&vid=B91E8C6D6FE990DB&iid=DF92D298D3FF1E6E&sid=9A3A88A3A0B8496C&eid=9438DF4A1CE63979&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=5&reference_num=19