%0 Journal Article
%T Analysis of porcine preadipocytes differentiation by atomic force microscope<br>应用原子力显微镜分析猪脂肪前体细胞的分化
%A Shengpu Li
%A Ruyi Shi
%A Qiulan Wang
%A Mu Wang
%A Rui Gan
%A Jie Pan
%A Jiye Cai
%A Shouquan Zhang
%A <br>李盛璞
%A 师如意
%A 王秋兰
%A 王牡
%A 甘睿
%A 潘洁
%A 蔡继业
%A 张守全
%J 生物工程学报
%D 2011
%I 
%X Abnormal changes during fat formation are closely related to the prevalence of many diseases. In order to understand the formation mechanism of fat, we used atomic force microscopy (AFM) to characterize the morphology and mechanical properties of porcine preadipocytes during the differentiation. Preadipocytes and adipocytes were different morphologically. The surface roughness of adipocytes was less than preadipocytes by detection of the ultrastructure. The mechanical properties of preadipocytes were changed during differentiation with AFM-based force spectroscopy. Preadipocytes were 20% higher than adipocytes in the adhesion force, stiffness and Young's modulus. Therefore, AFM analysis of membrane changes related to adipocytes formation provided quantitative data in the nanometer level for further studying the formation mechanism of the adipocytes.
%K atomic force microscopy
%K preadipocytes
%K adipocytes
%K ultrastructure
%K mechanical properties<br>原子力显微镜，脂肪前体细胞，成熟脂肪细胞，超微结构，机械性能
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=D6C382A3C22C0E5495A6E4821744EC04&yid=9377ED8094509821&vid=DB817633AA4F79B9&iid=CA4FD0336C81A37A&sid=2F56B21F91C9B05B&eid=B47A0E731AF43EB2&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=1&reference_num=18