%0 Journal Article
%T Progress on biodiesel production with enzymatic catalysis in China<br>酶法合成生物柴油工业化研究进展
%A Tianwei Tan
%A Jike Lu
%A Kaili Nie
%A Haixia Zhang
%A Li Deng
%A Fang Wang
%A <br>谭天伟
%A 鲁吉珂
%A 聂开立
%A 张海霞
%A 邓利
%A 王芳
%J 生物工程学报
%D 2010
%I 
%X This paper reports the progress of biodiesel production with enzymatic catalysis in Beijing University of Chemical Technology, one of the leaders in biodiesel R & D in China, which includes screening of high-yield lipase production strains, optimization and scale-up of the lipase fermentation process, lipase immobilization, bioreactor development and scale-up, biodiesel separation and purification and the by-product glycerol utilization. Firstly, lipase fermentation was carried out at industrial scale with the 5 m3 stirred tank bioreactor, and the enzyme activity as high as 8 000 IU/mL was achieved by the species Candida sp. 99-125. Then, the lipase was purified and immobilized on textile membranes. Furthermore, biodiesel production was performed in the 5 m3 stirred tank bioreactor with an enzyme dosage as low as 0.42%, and biodiesel that met the German biodiesel standard was produced. And in the meantime, the byproduct glycerol was used for the production of 1,3-propanediol to partly offset the production cost of biodiesel, and 76.1 g/L 1,3-propanediol was obtained in 30 L fermentor with the species Klebsiella pneumoniae.
%K biodiesel
%K enzymatic catalysis
%K lipase immobilization
%K 1
%K 3-propanediol co-production<br>生物柴油，酶催化转化，脂肪酶固定化，1
%K 3-丙二醇联产
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=47DFFBCB30A46439740C76DCEC62AF71&yid=140ECF96957D60B2&vid=96C778EE049EE47D&iid=DF92D298D3FF1E6E&sid=6484E0C1B87D264C&eid=0FCE62EC9DC00F5E&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=2&reference_num=0