%0 Journal Article
%T Construction and Analysis of Subtractive cDNA Library of Phellodendron amurense Under Drought Stress<br>干旱胁迫下黄檗幼苗cDNA消减文库的构建和分析
%A Huimei Wang
%A Yanbing Wang
%A Yuangang Zu
%A Lianhui Sun
%A <br>王慧梅
%A 王延兵
%A 祖元刚
%A 孙莲慧
%J 生物工程学报
%D 2008
%I 
%X With cDNA from Phellodendron amurense seedlings treated with drought stress as tester and cDNA from this plant in normal growth as driver, we construct cDNA subtracted library using suppression subtractive hybridization (SSH). In the library, the rate of recombination was 95%, the size of inserts was 300~800 bp. Two hundred and sixty-five new genes were obtained by DNA sequencing 816 positive clones picked randomly, and partitioned to 16 classes after nucleotide Blast and BlastX homological analysis against NT, NR, SWISSPROT, KEGG database. Forty-four drought stress associated genes, such as heat shock protein cognate 70, dehydration responsive protein 22, universal stress protein, metallothionein II, late embryogenesis abundant protein, were obtained, which made 16.6% of the overall genes. These genes included osmotic regulator, signal component regulatory protein and antioxidant enzyme. The research had established a basis for cloning stress resistance genes and further studying genes expression in P. amurense seedlings under drought stress.
%K drought stress
%K Phellodendron amurense
%K suppression subtractive hybridization
%K expressed sequence tags<br>干旱胁迫
%K 黄檗
%K 抑制性消减杂交
%K EST序列分析
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=8AF050EC61FE794B5BDC5A7D3A25B6B7&yid=67289AFF6305E306&vid=B91E8C6D6FE990DB&iid=0B39A22176CE99FB&sid=FEF02B4635FE8227&eid=974CBB04624305A1&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=1&reference_num=13