%0 Journal Article
%T Construction of Selectable Marker-removable Plant Expression Vectors<br>选择标记可去除的植物高效表达载体的构建
%A GAO Shang
%A SU Lei
%A JIA Hong-Ge
%A GUO Hong-Nian
%A GUO Hong-Nian
%A FANG Rong-Xiang
%A CHEN Xiao-Ying
%A <br>高尚
%A 苏鸓
%A 贾洪革
%A 郭洪年
%A 田颖川
%A 方荣祥
%A 陈晓英
%J 生物工程学报
%D 2007
%I 
%X The commonly used plant constitutive expression vector pBI121 was modified by insertion of two directly orientated lox sites each at one end of the selectable marker gene NPTII and by replacing the GUS gene with a sequence composed of multiple cloning sites (MCS). The resulting plant expression vector pBI121-lox-MCS is widely usable to accommodate various target genes through the MCS, and more importantly to allow the NPTII gene removed from transformed plants upon the action of the Cre recombinase. In addition, the CaMV 35S promoter located upstream of the MCS can be substituted with any other promoters to form plant vectors with expression features specified by the introduced promoters. Provided in this paper is an example that an enhanced phloem-specific promoter of the pumpkin PP2 gene (named dENP) was used to construct an NPTII-removable phloem-specific expression vector pBdENP-lox-MCS. Moreover, to facilitate screening of selectable marker-removed transgenic plants, we constructed another vector pBI121-gfp-lox-MCS in which a gfp-expression cassette is linked to the NPTII gene and the composite sequence is flanked by lox sites. Thus the selectable marker-free plants can be visually identified by loss of GFP fluorescence. The above newly created plant expression vectors can be used to develop selectable marker-removable transgenic plants for a variety of purposes.
%K plant expression vectors
%K selectable marker-removal
%K tissue-specific expression promoter
%K Cre/loxP DNA recombination system<br>植物表达载体
%K 去除选择标记
%K 组织特异性表达启动子
%K Cre/loxP
%K DNA重组系统
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=AACFA0B458437207&yid=A732AF04DDA03BB3&vid=EA389574707BDED3&iid=CA4FD0336C81A37A&sid=0B4F496D54044D86&eid=1B97AE5098AEB49C&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=17