%0 Journal Article
%T Extracellular expression of protein elicitor PeaT1 in Bacillus subtilis to enhance drought tolerance and wheat growth<br>蛋白激发子PeaT1在枯草芽胞杆菌中的分泌表达及重组菌株提高小麦抗旱和促生的作用
%A Limei Wang
%A Xiufen Yang
%A Hongmei Zeng
%A Dewen Qiu
%A Lihua Guo
%A Zheng Liu
%A <br>王立梅
%A 杨秀芬
%A 曾洪梅
%A 邱德文
%A 郭立华
%A 刘峥
%J 生物工程学报
%D 2011
%I 
%X PeaT1, a protein elicitor from Alternaria tenuissima can promote plant growth and trigger systemic acquired resistance in plants. In order to expand the application of PeaT1, P43 promoter sequence and nprB signal peptide-encoding sequence were cloned from Bacillus subtilis 168 chromosomal DNA. The two sequences and peaT1 gene were spliced by overlapping extension. This product was cloned into the Escherichia coli-B. subtilis shuttle vector pHY300-PLK and the resultant recombinant expression vector (pHY43N- peaT1) plasmid was transformed into B. subtilis WB800. SDS-PAGE and Western blotting analysis showed that protein elicitor PeaT1 was expressed extracellularly in B. subtilis. This recombinant bacterial strain enhanced drought tolerance and promoted seedling growth in wheat.
%K protein elicitor
%K Bacillus subtilis
%K extracellular expression
%K recombinant strain<br>蛋白激发子，枯草芽胞杆菌，分泌表达，重组菌
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=A66E90C274451689E69F6F0291467824&aid=F437B1BF01E7F486A51BFEF2BA8E9E5C&yid=9377ED8094509821&vid=DB817633AA4F79B9&iid=9CF7A0430CBB2DFD&sid=B7AB8E33F0FC19ED&eid=6300C37F864D5DEA&journal_id=1000-3061&journal_name=生物工程学报&referenced_num=0&reference_num=23